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Home page > Research interest > Recoding to study translation > Stop codon readthrough

Stop codon readthrough

For the organisms using a standard genetic code, translation termination occurs when one of the three stop codons, UAA, UGA and UAG, enters the ribosomal A-site. In contrast to the recognition of sense codons by tRNA, stop codons are recognized by extra-ribosomal proteins called class I release factors. The efficiency of translation termination depends on competition between the recognition of the stop codon by a class I release factors, and the decoding of stop codon by a near-cognate tRNAs.

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termination
Schematic of the competition between release factors and near-cognate tRNA.
 [1] Copyrigth 2010, Namy. [1] Translational errors: from yeast to new therapeutic targets. Bidou L, Rousset JP, Namy O. FEMS Yeast Res. 2010. (8):1070-82.

 

 

 

Translation termination mechanisms

Eukaryotic release factors In eukaryotes, two release factors eRF1 and eRF3 mediate translation termination. Either full or partial X-ray structures are available for both proteins and provide interesting insights into their function.

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Therapeutics approaches

Termination gains therapeutics interest One third of inherited diseases result from premature termination codon (PTC) mutations. These mutations lead to the synthesis of non-functional truncated polypeptides. In 1999, re-expression of the full-length dystrophin protein in a mouse model of muscular dystrophy was achieved by aminoglycoside treatment, resulting in a PTC within the coding sequence being bypassed. Since this seminal work, translation termination for treatment purposes has (...)

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